bio-protocol Webinar about Expansion Microscopy (ExM)
Next-generation expansion microscopy: 20x magnification in one step
Abstract
Expansion microscopy (ExM) has revolutionized biological imaging by enabling nanoscale-resolution visualization on conventional microscopes. Traditional ExM methods have been limited to ~4–10× expansion per step or require iterative processes to achieve higher magnifications (~15–20×). In this webinar, we will introduce 20ExM, a novel single-shot ExM protocol that achieves ~20× expansion in a single step, providing <20 nm resolution. This innovation simplifies high-resolution imaging while supporting postexpansion biomolecular staining in brain tissue. The discussion will cover the principles of ExM, the advantages of 20ExM over iterative expansion, and its potential applications in neuroscience, cell biology, and beyond.
Highlights
- Fundamentals of expansion microscopy (ExM) and its impact on high-resolution imaging
- 20ExM: A single-shot expansion protocol achieving ~20× linear expansion
- Post-expansion antibody staining for brain tissue and its implications
- Applications of 20ExM in neuroscience and cell biology and comparison with previous ExM approaches
- Q&A session
More Information
Speaker: Shiwei Wang B.A. PhD Candidate Massachusetts Institute of Technology
Certificates of attendance are available upon request (at least 30 minutes of LIVE webinar attendance required)
When: May 20, 2025 11:00 AM-12:00 PM EST
Register now
Source: https://bio-protocol.org/webinar (09.05.2025)